FIGURE 2.

Effect of low concentrations of HIV PI on virus properties. HIV particles were prepared from NL4-3-infected MT-4 cells incubated at the indicated concentrations of LPV. A and B, influence on Gag processing. Particle lysates were separated by SDS-PAGE (15%; acrylamide/bisacrylamide, 200:1), and Gag-derived proteins were detected (A) and quantified (B) by quantitative immunoblot using polyclonal antiserum against CA. C, infectivity of virus preparations. Virus samples from A were tested for single round infectivity on TZM-bl indicator cells as well as for infectivity on C8166 cells by TCID₅₀ titration. The graphs show relative infectivities normalized to the DMSO control (lane 1 in A) Error bars represent the standard deviation from three different dilutions. Note that relative titers on C8166 cells are plotted on a logarithmic scale although all other sales are linear. D, virus-associated reverse transcriptase. The amount of mature RT in particles (p66 and p51; black bars) in virus preparations from A was determined by quantitative immunoblotting. Particle-associated RT activity (gray bars) was measured in vitro using exogenous substrate. Error bars represent the standard deviation from three different dilutions.