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. 2009 Sep 2;284(43):29725–29734. doi: 10.1074/jbc.M109.042143

FIGURE 5.

FIGURE 5.

AhR expression and DNA strand break formation in AhR siRNA-transfected and nontransfected H358 cells treated with B[a]P-7,8-dione. H358 cells were transfected with AhR-siRNA and then treated with B[a]P-7,8-dione for 3, 6, or 12 h. The cells were then harvested and were divided into two aliquots. One was used for Western blot analysis, and other was used for the hOGG1-coupled comet assay. A, AhR expression in both AhR siRNA-transfected and nontransfected H358 cells treated with B[a]P-7,8-dione. Lane 1, non-AhR siRNA-transfected cells; lane 2, nontransfected cells treated with B[a]P-7,8-dione; lane 3, AhR-siRNA transfected cells; lane 4, AhR siRNA-transfected cells-treated with B[a]P-7,8-dione. B, AhR-dependent DNA strand break formation in AhR siRNA-transfected H358 cells treated with DMSO. C, AhR-dependent DNA strand break formation in AhR siRNA-transfected H358 cells treated with B[a]P-7,8-dione. Significant effects were observed after hOGG1 treatment. *, p < 0.05. Significant effects was observed between AhR siRNA-transfected cells and nontransfected cells-treated with B[a]P-7,8-dione for 6 h. *, p < 0.05. A detailed description can be found under “Experimental Procedures.” WT, wild type; AhR-k/d, aryl hydrocarbon receptor knock down cells; AU, arbitrary units.