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. 2009 Sep 2;284(43):29725–29734. doi: 10.1074/jbc.M109.042143

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — LC/MS detection of 8-oxo-dGuo in AhR siRNA-transfected and nontransfected H358 cells-treated with B[a]P-7,8-dione. AhR siRNA-transfected H358 cells and nontransfected H358 cells were treated with B[a]P-7,8-dione for 6 h. The cellular DNA was then extracted and digested. The levels of 8-oxo-dGuo in samples were determined by LC-ESI/MS/MRM. A, LC/MS chromatograms of 8-oxo-dGuo in AhR siRNA-transfected H358 cellular DNA. The upper two panels show ion-chromatograms for the detection of endogenous 8-oxo-dGuo and the deuterated [¹⁵N₅]8-oxo-dGuo internal standard obtained from parental H358 cells-treated with B[a]P-7,8-dione. The lower two panels show ion chromatograms for the detection of 8-oxo-dGuo and the heavy isotope [¹⁵N₅]8-oxo-dGuo internal standard obtained from AhR siRNA-transfected H358 cells treated with B[a]P-7,8-dione. B, levels of 8-oxo-dGuo in both AhR siRNA-transfected and nontransfected H358 cells-treated with B[a]P-7,8-dione. Significant effects were found between AhR siRNA-transfected H358 cells and nontransfected H358 cells. *, p < 0.05. A detailed description can be found under “Experimental Procedures.”