FIGURE 1.

Effect of sI/R on grp78 mRNA and protein, pgk mRNA, and HIF-1α protein. A, cultured cardiac myocytes were subjected to sI for the times shown. The levels of rat grp78, pgk, and gapdh (glyceraldehyde-3-phosphate dehydrogenase) mRNA were assessed by reverse transcription quantitative PCR. Shown are the mean values of grp78 or pgk/gapdh mRNA expressed as -fold control level (control = 0 h sI/0 h sR) ± S.E. *, #, and §, p ≤ 0.05 different from control and all other values. B, cultured cardiac myocytes were treated with or without sI for 8 or 20 h, and then extracts were examined for rat HIF-1α and GAPDH by immunoblotting. The migration positions of 112-, 60-, and 37-kDa molecular mass markers are shown, as is the approximate expected migration position for full-length HIF-1α (n = 3 cultures/treatment). C, cultured cardiac myocytes were subjected to 20 h of sI/R for the times shown. The levels of rat grp78 and gapdh mRNA were assessed by reverse transcription quantitative PCR. Shown are the mean values of grp78/gapdh mRNA expressed as -fold control (control = 0 h sI/0 h sR) ± S.E. *, #, and §, p ≤ 0.05 different from control and all other values. D, cultured cardiac myocytes were subjected to sI or sI/R for the times shown. The levels of rat GRP78 and GAPDH were determined by immunoblotting (n = 3 cultures/treatment). The GRP78 levels were normalized to GAPDH and are shown as -fold control ± S.E.