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. 2009 Aug 11;284(43):29757–29772. doi: 10.1074/jbc.M109.038513

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Absorption spectra of Te-PixJ(GAF) variants under denaturing conditions. Purified Te-PixJ(Cyc-GAF), the D492H, C494A, and C522A mutants, and wild-type SyB-Cph1(GAF) were assembled with PCB and then denatured in acidic urea in either their dark-adapted states (Pb for Te-PixJ and Pr for SyB-Cph1) or their excited states (Pg for Te-PixJ and Pfr for SyB-Cph1). UV-visible absorption spectra were measured either in darkness (solid lines) or following irradiations with light near their peak absorption (dashed lines). Free PCB was included for comparison. Absorption maxima are indicated.