Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 11;284(43):29757–29772. doi: 10.1074/jbc.M109.038513

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — NMR spectroscopy of Te-PixJ(Cyc-GAF) assembled with [¹⁵N]PCB or [¹³C]PCB. A, proposed structure of PVB bound via Cys-494 and Cys-522. The filled circles locate the carbons labeled with ¹³C. B, two-dimensional ¹H-¹⁵N HSQC NMR spectra of [¹⁵N]PCB-labeled Te-PixJ(GAF) before (Pb) and after (Pg) irradiation with saturating blue light. The arrows highlight the ¹⁵N chemical shifts that change position upon Pb → Pg photoconversion. C, one-dimensional ¹³C and two-dimensional ¹H-¹³C NMR spectra of [¹³C]PCB-labeled Te-PixJ(Cyc-GAF) in the methine region of the spectra before (Pb) and after (Pg) irradiation with saturating blue light. D, one-dimensional ¹³C NMR spectra of [¹³C]PCB-labeled Te-PixJ(Cyc-GAF) before (Pb) and after (Pg) irradiation with saturating blue light in the CO region of the spectra (170–180 ppm).

Inline graphic — NMR spectroscopy of Te-PixJ(Cyc-GAF) assembled with [¹⁵N]PCB or [¹³C]PCB. A, proposed structure of PVB bound via Cys-494 and Cys-522. The filled circles locate the carbons labeled with ¹³C. B, two-dimensional ¹H-¹⁵N HSQC NMR spectra of [¹⁵N]PCB-labeled Te-PixJ(GAF) before (Pb) and after (Pg) irradiation with saturating blue light. The arrows highlight the ¹⁵N chemical shifts that change position upon Pb → Pg photoconversion. C, one-dimensional ¹³C and two-dimensional ¹H-¹³C NMR spectra of [¹³C]PCB-labeled Te-PixJ(Cyc-GAF) in the methine region of the spectra before (Pb) and after (Pg) irradiation with saturating blue light. D, one-dimensional ¹³C NMR spectra of [¹³C]PCB-labeled Te-PixJ(Cyc-GAF) before (Pb) and after (Pg) irradiation with saturating blue light in the CO region of the spectra (170–180 ppm).