FIGURE 6.

Two-dimensional ¹H-¹⁵N HSQC NMR spectra of [¹⁵N]Te-PixJ(Cyc-GAF) in the Pb and Pg states. NMR data were collected with the C555A variant of Te-PixJ(Cyc-GAF) with the polypeptide uniformly labeled with ¹⁵N and assembled with unlabeled PCB. Blue, NMR spectrum of Pb. Green, NMR spectrum of Pg. Magenta, anti-alias chemical shifts. The inset shows a magnification of a region in the ¹H-¹⁵N HSQC spectra that includes a number of peaks that substantially change position upon Pb to Pg photoconversion.