FIGURE 7.

IR and RR spectroscopy of Te-PixJ(Cyc-GAF). A, experimental Pg-Pb difference IR spectra as compared with calculated IR spectra for various chromophore variants. PVB-C4, PVB-C5, and PCB-C10 denote PVB and PCB variants bound via the C4, C5, and C10 carbons, respectively. B, experimental Pg-Pb and calculated (ZZEssa-ZZZssa) IR difference spectra for chromophores with protonated (solid line) and deuterated (dashed line) pyrrole nitrogens (top two spectra) and for the natural abundance (solid line) and the ¹³C-labeled (dashed line) chromophores (bottom two spectra). For the location of the ¹³C-labeled carbons, see Fig. 5A. Solid lines = H₂O or ¹²C samples. The calculated spectra refer to the PVB chromophore. C, experimental RR spectra of the Pb and Pg states as compared with calculated RR spectra of PVB in the ZZZssa and ZZEssa geometries. Solid and dashed lines refer to chromophores protonated and deuterated at the pyrrole nitrogens, respectively.