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. 2009 Aug 11;284(43):29757–29772. doi: 10.1074/jbc.M109.038513

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Absorption and fluorescence properties of Te-PixJ(Cyc-GAF) mutants. A, photographs of purified solutions of Te-PixJ(Cyc-GAF) and the mutants in white light (WL) or during irradiation with UV light. Samples were kept in the dark prior to photography. Syn-Etr1(Cyc-GAF) is included for comparison. B, absorption spectra of the Te-PixJ(Cyc-GAF) mutants affecting Asp-492. C, fluorescence excitation (dashed lines) and emission spectra (solid lines) of a subset of Te-PixJ(Cyc-GAF) variants shown in panel A. Excitation and emission maxima are indicated. The fluorescence spectra of the D86H mutant of SyB-Cph1(GAF) are included for comparison.