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. 2009 Jul 28;284(43):29798–29808. doi: 10.1074/jbc.M109.029470

FIGURE 1.

FIGURE 1.

Thiazolides inhibit influenza A virus replication acting at a post-entry level. A, structure of NTZ and TIZ. B, NTZ (blue circles) and TIZ (red circles) inhibit the replication of mammalian (PR8 and WSN) and avian (A/Ck) influenza A virus strains in MDCK cells. Virus yield was determined at 24 h p.i. C, antiviral activity of TIZ on influenza A PR8 virus in human monocytic U937 (●) and T-lymphoblastoid Jurkat (▴) cells and WSN virus in human lung epithelial A549 cells (♦). D, MDCK cells were treated with 10 μg/ml TIZ (filled bars) at the indicated times before infection (Pre), immediately after the adsorption period (Post), or only during the adsorption period (Ad, dashed bar). The empty bar represents untreated infected control (C). E, long term antiviral activity of TIZ in PR8-infected MDCK cells treated with 10 μg/ml TIZ (filled circles) or vehicle (empty circles) after virus adsorption. B–E, virus yield, expressed in HAU/ml (B and E) or as a percentage of untreated control (C and D), represents the means ± S.D. of duplicate samples from a representative experiment of three with similar results. *, p < 0.01; **, p < 0.05.