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. 2009 Sep 1;284(43):29817–29827. doi: 10.1074/jbc.M109.050187

FIGURE 1.

FIGURE 1.

Confocal microscopy of co-transfected βarr2-GFP and GPR55. A, transiently transfected βarr2-GFP (in cytoplasm, see the arrow) and GPR55 in untreated HEK293 cells. B and C, 10 μm LPI induces redistribution of βarr2-GFP in HEK 293 cells expressing GPR55 (B) and HA-GPR55E (C). D and E, corresponding GFP and Alexa Fluor 568 fluorescence channels of a field of U2OS cells stably transfected with βarr2-GFP and HA-GPR55E. D, under basal conditions the βarr2-GFP is uniformly distributed in the cell cytoplasm. Panel E shows the plasma membrane staining of HA-GPR55E in these cells using anti-HA epitope mouse primary antibody and goat anti-mouse Alexa Fluor 568 secondary antibody. F depicts the overlay of panel D and E. G, cells as in E, in which treatment with primary anti-HA antibody is omitted. No membrane staining of receptor is observed in the Alexa Fluor 568 channel.