FIGURE 2.

Agonist induced trafficking of βarr2-GFP in U2OS cells containing GPR55E. The redistribution of βarr2-GFP fluorescence was visualized after a 40-min treatment with 3 μm LPI (A), 30 μm SR141716A (C), and 30 μm AM251 (E). B, D, and F represent the concentration-response curves for LPI, SR141716A, and AM251, respectively. G shows the cytosolic distribution of βarr2-GFP in vehicle-treated cells. 4′,6-diamidino-2-phenylindole nuclear staining (arrows) shows nuclear exclusion of βarr2. H, the maximum number of β-arrestin aggregates (objects) was determined from the calculated plateaus of the concentration-response curves for each compound to determine relative efficacies. The data represent the mean ± S.E. from at least three independent experiments where triplicate images of fields containing multiple cells were analyzed. Representative images that were captured at 40× magnification are depicted.