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Ligand induced trafficking of βarr2-GFP in U2OS cells containing CB1RE, a receptor with a phosphorylation site-enhanced C-tail. Shown are representative live cell confocal images of a line of U2OS cells permanently transfected with CB1E receptors and β2-arrestin GFP. Cells were treated for 40 min at 37 °C with media containing vehicle (A), 10 μm LPI (B), 10 μm SR141716A (C), and 3 μm CP55,940 (D).
