FIGURE 3.

Sarcomere disorganization caused by Fhod3 knock down is restored by expression of the wild-type (wt) Fhod3 but not by that of mutant proteins defective in the actin assembly activity. A, cardiomyocytes were sequentially transfected with the adenovirus encoding HA-tagged wild-type or mutant Fhod3 and with Fhod3 siRNA 4 and cultured for 48 h. Cells were fixed and double stained with the antibodies against anti-HA (red) and α-actinin (green). Scale bar, 10 μm. B, cells with well developed sarcomere organization were counted, and the percentages from five independent transfections are expressed as the means ± S.D. as in Fig. 2C. *, p < 0.001; **, p < 0.005, Welch's t test. C, the protein level of exogenously expressed HA-tagged Fhod3 was determined by immunoblot analysis using the anti-HA antibody.