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Western blot of cell lysates from MDTF cells (A), MDTFPiT1-HA cells (B), and MDTFPiT1-13S97C-HA cells (C) under different labeling conditions. Lane 1, cells were labeled with B-mal; lane 2, cells were pretreated with MTSET and then labeled with B-mal; lane 3, cells were permeabilized with SLO before B-mal labeling; lane 4, cells were first treated with MTSET, then permeabilized with SLO, and finally labeled with B-mal.
