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. 2009 Aug 28;284(43):29979–29987. doi: 10.1074/jbc.M109.022566

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — A, representative Western blot of lysates of MDTF cells expressing PiT1-13-HA mutants containing individual cysteines L545C, Y546C, L547C, V548C, Y549C, D550C, V554C, and K557C. B, lysates of cells expressing above mutant proteins treated with MTSET prior to B-mal labeling. C, lysates of MDTF cells expressing PiT1-13-HA mutants containing individual cysteines R581C, L591C, and T592C reacted with B-mal. D, lysates of cells expressing above mutants permeabilized with SLO before B-mal labeling. PiT1-HA is the positive control for SLO.