FIGURE 3.

HNF4α is required for SIRT1 activator-induced transcriptional repression of PEPCK-C. Chinese hamster ovary cells were co-transfected with p2000-Luc, PKAc, and various amounts of plasmid overexpressing HNF4α. Cells were then treated with 5 mm IsoNAM or 20 μm resveratrol. The total amount of DNA for each transfection was adjusted to the same value by adding empty vector DNA. The results were expressed as the mean ± S.E. of triplicate measurements. *, p < 0.001 and **, p < 0.004. Insets show levels of HNF4α in cells as determined by Western blotting.