TABLE 1.
Cell | Mutation | Defect | Sensitivitya |
|
---|---|---|---|---|
FA | azadC | |||
CHO cells | ||||
AA8 | WT | None | Control | Control |
51D1 | RAD51D | HR | ++ | ++ |
51D1.3 | RAD51 + cDNAb | HR corrected | − | − |
irs1SF | XRCC3 | HR | +++ | +++ |
V3 | DNA-PKcs | NHEJ | − | − |
UV5 | ERCC2 (XPD) | NER | + | −c |
UV41 | ERCC4 (XPF) | NER | ++ | +d |
Human cells | ||||
WI38VA13 | WT | None | Control | Control |
XP12ROSV | XPA | NER | + | − |
XP2OSSV | XPA | NER | + | − |
XP6BESV | XPD | NER | + | − |
a Symbols denote sensitivity relative to WT cells for 0.3 mm FA or 2 μm azadC as follows: no sensitivity (−) and sensitivity increased by 1 order (+), 2 orders (++), and 3 orders (+++) of magnitude. Data were from survival curves in Fig. 2E for human cells and in supplemental Fig. S5 for CHO cells. Survival curves for V3 and XP6BESV cells are not shown.
b Complemented with hamster RAD51D cDNA.
c Slightly resistant (2.3-fold) relative to WT cells.
d Slightly sensitive (2.8-fold) relative to WT cells.