FIGURE 2.

Syndecan-2 is highly expressed in human skin melanoma cells. A, paraffin-embedded tissue sections were either stained with hematoxylin and eosin (H&E) (a) or hematoxylin (f and k) or immunostained with an anti-syndecan-2 antibody (SDC2) (b, g, and l). d, negative control for immunostaining with the preimmune serum. The immunoreactivities were visualized by 3,3′-diaminobenzidine (DAB) intensified with nickel. c, h, and m, higher magnifications of b, g, and l, respectively. Immunostaining with an anti-HMB-45 antibody was detected using the same methods (e, i, and n). j and o are higher magnifications of i and n. The arrowheads indicate the melanocyte with immunoreactivities of syndecan-2 and HMB-45, respectively. Representative images from one of five experiments are shown. B, cells from human melanoma cell lines were incubated with anti-syndecan-2 antibody, and protein expression level was analyzed by flow cytometry. IgG was used as the control.