TABLE 5.
Effect of high temperature on respiratory rate and cytochrome content in tps2Δ strains
The respiratory rate of cells of the wild type and the three mutant strains was measured during growth on YPGal2% at 35 °C at an optical density of 4 (spontaneous respiration). When added, as indicated in the table (uncoupled JO2), the ethanol concentration was 107 mm, and CCCP was 5 μm. The cytochrome, Tre6P, and cAMP contents were measured as described under “Experimental Procedures.” The values are the means ± S.E. of seven measurements made with different cultures. natO, nanoatoms of oxygen.
| Parameters | Wild type | tps2Δ | tps2Δ hxk2Δ | tps1Δ |
|---|---|---|---|---|
| Spontaneous JO2(natO/min/mg DW) | 187 ± 10 | 207 ± 9 | 118 ± 7a | 131 ± 11b |
| Uncoupled JO2(natO/min/mg DW) | 257 ± 16 | 364 ± 12a | 206 ± 8 | 202 ± 27 |
| cAMP (pmol/mg DW) | 1.9 ± 0.2 | 4.6 ± 0.2a | 1.5 ± 0.2 | 1.4 ± 0.4 |
| c + c1 (pmol/mg DW) | 44 ± 2 | 53 ± 5 | 41 ± 2 | 40 ± 4 |
| b (pmol/mg DW) | 27 ± 3 | 31 ± 4 | 25 ± 2 | 24 ± 3 |
| a + a3 (pmol/mg DW) | 6.0 ± 0.7 | 11 ± 1.3b | 4.2 ± 0.5 | 5 ± 1.8 |
| Tre6P (nmol/mg DW) | 3.1 ± 0.5 | 22.2 ± 7.3b | 3.3 ± 0.4 | 0.8 ± 0.4b |
a The differences between wild type and mutant strains were significant at p < 0.001.
b The differences between wild type and mutant strains were significant at p < 0.05.