FIGURE 6.
Geminin stabilizes Cdt1 after activation. Geminin sense or antisense oligos injected in in vitro matured oocytes were injected with CaCl2. At the indicated times samples were collected and analyzed for cyclin B1, Geminin, and Cdt1 expression by Western analysis. CSF is a positive control; Mcm4 is a loading control, and the asterisk indicates exogenous Geminin. Zero time point corresponds to mature oocyte (240 min after GVBD). A and B, activation in the absence of exogenous Geminin. C, activation in the presence of exogenous Geminin. Experiments carried out in A and B are from the same batch of oocytes, whereas that of C is on a different batch of oocytes. BSA, bovine serum albumin.