FIGURE 2.

Halo of Lewy bodies is fuzzy and contains ubiquitin, whereas the core consists of compacted α-synuclein. Transmission electron microscopic images of Lewy bodies without (top panel) and after proteinase K digestion (bottom panel). Lewy bodies were affinity-isolated with anti-α-synuclein antiserum from brain cortex and immunostained with anti-ubiquitin and anti-synuclein primary and gold particle-labeled secondary antibodies and counterstained with uranyl acetate before embedding. Bar, 0.25 μm.