FIGURE 2.

Different agonists induce distinct conformations in loop D in the inner β-sheet. A–C, current (black) and fluorescence (red) traces recorded from oocytes injected with Q67C during application of glycine (A), β-alanine (B), and taurine (C) (traces in A modified from Ref. 27). D, comparison of agonist-induced maximal current (I_max, gray), maximal fluorescence (ΔF_max, red), and current EC₅₀ (black). Data normalized to mean values obtained for glycine (27). Asterisks indicates significant difference to glycine values (*, p < 0.005, Student's t test). Double asterisks indicates significant difference to both glycine and β-alanine values (**, p < 0.05, Student's t test).