FIGURE 1.

Lauric acid enhances the recruitment of TLR4 and its adaptor molecules, TRIF and MyD88, into the lipid rafts. RAW264.7 cells were stimulated with LPS (100 ng/ml) or lauric acid (100 μm, C12) for 7 min or DHA (20 μm) for 1 h. The cells were lysed and lipid rafts (Fractions 2 and 3) and non-lipid rafts fractions (Fractions 5–8) were separated by sucrose-gradient ultracentrifugation. Each fraction was trichloroacetic acid precipitated and subjected to immunoblotting using anti-TLR4, anti-TRIF, anti-MyD88, or anti-flotillin-1 antibodies.