FIGURE 2.

CRMP2 is phosphorylated by Fyn in the region of 1–212. A, identification of the region of CRMP2 phosphorylated by Fyn. CRMP2 full-length or CRMP2 partial fragment 1–212, 231–572, or 415–572 was transfected into HEK293T cells with FynCA. The fragments were immunoprecipitated (IP) and subjected to immunoblotting with anti-phosphotyrosine antibody (PY-99) or anti-Myc antibody. The N-terminal region of CRMP2-(1–212) was well phosphorylated by Fyn. Asterisks indicate phosphorylated or intact bands of CRMP2. Arrowheads indicate nonspecific bands. The two bands in Myc blot of CRMP2-(213–572) and CRMP2-(415–572) may be caused by proteolysis. B, summary of the results shown in A.