FIGURE 2.

Nitric oxide fails to activate p53 in INS 832/13 cells or rat islets. INS 832/13 cells (A–C) or rat islets (D and E) were treated with IL-1 (10 units/ml) ± NMMA (2 mm) or DEANO (1 mm) for the indicated times. Following the treatments, culture supernatants were harvested, and nitrite production was determined (B and E). Cells were harvested, and phopsho-p53 and total p53 were determined by Western blot analysis. Camptothecin was used as a positive control for p53 activation, and GAPDH was used as a loading control. Results are representative of at least three experiments.