FIGURE 6.

GADD45α is essential for repair of nitric oxide-induced DNA damage. INS 832/13 cells were transfected with 30 nm GADD45α or scramble (negative control) siRNA as indicated. Forty-eight hours after transfection, INS 832/13 cells were treated with DEANO for 1 h or with DEANO for 1 h followed by washing to remove the nitric oxide donor, and continued culture for 5 additional h (repair conditions). A, the cells were harvested, and DNA damage was quantified by the comet assay. B, cells were also harvested, and real time PCR was used to confirm knockdown of GADD45α mRNA using siRNA. Results are the average ± S.E. of three independent experiments.