FIGURE 9.

Adipogenesis is not an inevitable accompaniment of increased lipid oxidation. A, OB-6γ2 cells were cultured to induce or repress PPARγ2 gene expression and then treated with vehicle (PBS), RGL (1 nm), or the indicated oxidized PUFAs (100 μm) for 8 days. Lipid was visualized with Oil Red O (inset, PPARγ2-expressing cells) and quantified as described under “Experimental Procedures.” *, p < 0.05 versus vehicle control. †, p < 0.05 versus RGL by ANOVA. B, adipocytes (arrow, left panel) in calvaria bone of B6 mice were quantified by histomorphometry (middle panel) of hematoxylin- and eosin-stained decalcified sections. Quantification of the adipocyte marker Fabp4 was done by quantitative PCR after normalization to glyceraldehyde-3-phosphate dehydrogenase in a separate experiment (right panel). C, adipocytes in toluidine blue-stained nondecalcified sections (left panel) and Fabp4 expression (right panel) number was determined in vertebral bone as in B. Bars represent the means ± S.D.; *, p < 0.05 versus 4 or 6 months.