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. 2009 Aug 3;284(40):27533–27543. doi: 10.1074/jbc.M109.048884

FIGURE 5.

FIGURE 5.

Involvement of CREB in Wnt-5a- and DARPP-32-mediated inhibition of cell migration. A, Western blot showing rWnt-5a-induced CREB phosphorylation in MCF-7 cells, as detected by anti-pCREB antibody. B, Western blot showing CREB phosphorylation in MCF-7 cells transfected with empty vector or Myc-DARPP-32 expression plasmid, non-stimulated or stimulated with 0.4 μg/ml rWnt-5a for or 1 μm forskolin for 10 min. Each Western blot is representative of six independent experiments. C, wound-healing assay with MCF-7 cells in collagen-coated plates, transfected with either empty vector, Myc-DARPP-32 vector, or both Myc-DARPP-32 and DN-CREB vectors. All cells were stimulated with 0.4 μg/ml rWnt-5a for 24 h (n = 12). D, migration of MCF-7 through collagen-coated wells transfected with empty vector, Myc-DARPP-32 vector, or both Myc-DARPP-32 and DN-CREB vectors (n = 6).