Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 5;284(40):27544–27556. doi: 10.1074/jbc.M109.042036

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — MET30 has zinc- and Zap1-responsive gene expression. A, proposed regulatory circuitry controlling MET3, MET14, and MET16 expression in response to zinc. B, transcriptional regulation of MET30 in response to zinc and Zap1 activity. Wild type (DY1457) cells were grown in LZM supplemented with a range of added zinc, and mRNA levels of MET30 and CMD1 were analyzed by S1 nuclease protection assay (lanes 1–7). In addition, wild type and zap1Δ mutant cells (ZHY6) were grown in low zinc (−Zn, LZM + 1 μm ZnCl₂) (lanes 8 and 9), and wild type cells with and without expressing the constitutive Zap1^TC allele were grown in zinc-replete SD medium (+Zn, lanes 10 and 11). RNA was isolated and analyzed by S1 nuclease protection assay. C, met4Δ mutant strain (TAL31) was grown in LZM supplemented with methionine and with a range of added zinc. RNA was isolated and analyzed by S1 nuclease protection assay.