TABLE 1.
Genes potentially repressed by Zap1
| ORF | Genea | Function | Fold repressionb |
|||
|---|---|---|---|---|---|---|
| E3+ Zn; WT/Zap1TC |
E4 WT; +Zn /−Zn |
|||||
| Exp. A | Exp. B | Exp. A | Exp. B | |||
| Zinc homeostasis | ||||||
| YOL086C | ADH1 | Alcohol dehydrogenase I | 2.5 | 2.1 | 4.1 | 3.3 |
| YMR083W | ADH3 | Alcohol dehydrogenase III | 2.9 | 2.5 | 1.9 | 1.9 |
| YMR303C | ADH2 | Alcohol dehydrogenase II | 2.9 | 2.0 | 4.3 | 3.4 |
| Sulfur metabolism | ||||||
| YGL254W | FZF1 | Transcription factor involved in sulfite metabolism | 1.6 | 1.6 | 1.6 | 1.6 |
| YJR010W | MET3 | Sulfate adenylyltransferase, sulfate assimilation | 2.3 | 2.1 | 1.6 | 1.5 |
| YKL001C | MET14 | Adenylylsulfate kinase, sulfate assimilation | 2.3 | 2.3 | 1.9 | 2.1 |
| YPR167C | MET16 | Phosphoadenylyl-sulfate reductase, sulfate assimilation | 2.1 | 2.0 | 1.6 | 2.0 |
| Mitochondrial function | ||||||
| YDR194C | MSS116 | RNA helicase, mitochondrial RNA splicing | 1.8 | 1.7 | 1.5 | 1.6 |
| YJL166W | QCR8 | Ubiquinol-cytochrome c reductase | 1.7 | 1.6 | 1.5 | 1.6 |
| YJR122W | CAF17 | Mitochondrial protein, function unknown | 1.6 | 1.7 | 1.5 | 1.5 |
| Nucleotide metabolism | ||||||
| YJR025C | BNA1 | 3-Hydroxyanthranilic acid dioxygenase, nicotinic acid synthesis | 2.2 | 1.9 | 2.6 | 2.4 |
| YPR062W | FCY1 | Cytosine deaminase, uracil synthesis | 1.8 | 1.7 | 1.6 | 1.6 |
| Amino acid metabolism | ||||||
| YDR380W | ARO10 | Phenylpyruvate decarboxylase | 2.7 | 2.7 | 1.6 | 1.5 |
| YGL009C | LEU1 | 3-Isopropylmalate dehydratase, leucine synthesis | 1.8 | 1.7 | 3.2 | 3.1 |
| YHR208W | BAT1 | Transaminase, branched-chain amino acid synthesis and degradation | 2.3 | 2.2 | 2.2 | 2.5 |
| YPR060C | ARO7 | Chorismate mutase, aromatic amino acid synthesis | 1.7 | 1.5 | 1.5 | 1.6 |
| Protein synthesis | ||||||
| YDR471W | RPL27B | Large (60 S) ribosomal subunit protein | 2.2 | 2.6 | 1.4 | 1.6 |
| YER056C-A | RPL34A | Large (60 S) ribosomal subunit protein | 1.8 | 2.0 | 1.4 | 1.7 |
| YGR084C | MRP13 | Mitochondrial small ribosomal subunit protein | 1.7 | 1.9 | 1.5 | 1.5 |
| YLR448W | RPL6B | Large (60 S) ribosomal subunit protein | 2.0 | 1.9 | 1.4 | 1.6 |
| YML073C | RPL6A | Large (60 S) ribosomal subunit protein | 1.9 | 1.9 | 1.4 | 1.6 |
| YOL040C | RPS15 | Small (40 S) ribosomal subunit protein | 2.3 | 1.9 | 1.7 | 2.1 |
| YOR091W | TMA46 | Ribosome-associated protein | 1.7 | 1.6 | 1.5 | 1.6 |
| Others | ||||||
| YBR158W | CST13 | Protein required for daughter cell separation | 1.6 | 1.5 | 1.5 | 1.8 |
| YDR177W | UBC1 | E2 ubiquitin-conjugating enzyme | 1.6 | 1.6 | 1.5 | 1.5 |
| YEL040W | UTR2 | Cell wall protein, putative chitin transglycosidase | 2.5 | 2.1 | 2.3 | 2.5 |
| YGL089C | MFα2 | α-Factor mating pheromone | 3.1 | 2.8 | 9.3 | 8.7 |
| YIL118W | RHO3 | Small GTPase protein, control of cell polarity | 1.8 | 1.7 | 1.6 | 1.5 |
| YIL158W | Function unknown | 1.6 | 1.6 | 1.4 | 1.6 | |
| YKL029C | MAE1 | Malate dehydrogenase | 2.0 | 1.6 | 1.6 | 1.7 |
| YKL178C | STE3 | α-Factor mating pheromone receptor | 2.1 | 2.2 | 2.0 | 1.8 |
| YML040W | Ty1 Gag nucleocapsid protein | 1.9 | 2.0 | 1.4 | 1.8 | |
| YMR051C | Ty1 Gag nucleocapsid protein | 2.3 | 2.3 | 1.4 | 1.6 | |
| YNL051W | COG5 | Involved in intra-Golgi vesicle trafficking | 1.9 | 1.6 | 2.4 | 2.1 |
| YNL255C | GIS2 | Involved in the RAS/cAMP signaling pathway | 2.6 | 2.3 | 2.7 | 2.5 |
| YOR315W | SFG1 | Putative transcription factor for pseudohyphal growth | 1.7 | 1.8 | 2.4 | 2.4 |
a Results obtained for the genes in boldface type were confirmed independently by S1 nuclease protection assay in Fig. 2.
b Fold repression is reported for each gene in duplicate experiments A and B.