FIGURE 2.

EDA1 activates NF-κB in HaCat keratinocytes. Panel A, HaCat keratinocytes were stained with Fc-EDA1-E245 (EDA1), in the presence or absence of EDAR-COMP-Flag (sol. EDAR) or BCMA-COMP-Flag (sol. BCMA), and bound Fc-EDA1 was monitored by FACS. Panel B, HaCat cells were stimulated with 200 ng/ml of Fc-TNF (TNF) or 200 ng/ml of Fc-EDA1-E245 (EDA1), for the indicated period of time. Cell extracts were analyzed by Western blotting for the presence of total IκBα (IκBα), phosphorylated IκBα (P-IκBα), or the loading control tubulin.