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Size exclusion chromatography of EDA1 and FasL with or without collagen domains. The indicated Flag-tagged proteins were fractionated by size exclusion chromatography on a Superdex 200 column. Active ligands were detected in the eluted fractions by means of a receptor-binding ELISA. The elution position of molecular mass markers (in kDa) is indicated at the top of the elution profile. The analysis of the data is shown in Table 1.
