FIGURE 7.

Differential effects of the collagen domain and HSPG-binding domain of EDA1 in vitro and in vivo. Panel A, Jurkat EDAR:Fas cells were treated with different Flag-EDA1 at the indicated concentration as described in the legend to Fig. 4. Panel B, EDA-deficient Tabby mice were treated ip at day 1 after birth with the indicated dose of the same Flag-EDA1 proteins used in panel A. Presence of hair on the tails was scored 3–4 weeks later (Score 0: no hair, like Tabby; 0.5: very few hair; 1: sparse hair on ventral side; 2: numerous hair on ventral side; 3: numerous hair on both sides; 4: dense hair on both sides, like wild type). Pictures of the tail of three mice per group treated at the dose of 2.4 mg/kg are shown on the right. The ventral and dorsal sides of the tail are facing left and right, respectively.