TABLE 1.
Sizes of EDA1 and FasL with or without collagen domains by size exclusion chromatograpy
| Ligand | Elution volume | Apparent size | Theoretical monomer molecular massa | Number of globular subunitsb | Estimated number of subunitsc |
|---|---|---|---|---|---|
| ml | kDa | KDa | |||
| FasL | 14.6 | 78 | 25.7 | 3.0 | 3.0 |
| ACRP-FasL | 11.25 | 447 | 35.1 | 12.7 | 6.4 |
| EDA1-S160–242:FasL | 11.85 | 327 | 33.7 | 9.7 | 4.8 |
| EDA1-E245 | 15 | 63 | 19.7 | 3.2 | 3.2 |
| EDA1-S160 | 12 | 302 | 29.1 | 10.4 | 5.2 |
| EDA1-S160 KKKGKK → SASGAS | 11.5 | 392 | 27.3 | 14.4 | 7.2 |
| ACRP-EDA1-E245 | 11.25 | 447 | 27.3 | 16.4 | 8.2 |
a Theoretical molecular mass of the mature polypeptide + 7.5 kDa (for three N-linked glycans of FasL) or + 1.3 kDa (for one N-linked glycan on half of the EDA1).
b Apparent size divided by the theoretical monomer molecular mass.
c Number of globular subunits divided by 2 if the molecule contains a collagen domain.