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. 2009 Aug 12;284(40):27577–27586. doi: 10.1074/jbc.M109.049361

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Functional studies showed the critical role of NRF-1 in the regulation of the VSNL1 promoter. A and B, DN NRF-1 inhibited the VSNL1 gene promoter. Different amounts of pCDNA3.1 Myc-DN-NRF-1 were co-transfected into NCI-H522 (A) and NCI-H520 (B) cells together with pGL4.10 VP-174 reporter, luciferase activity was normalized to vector (pCDNA3.1 Myc) transfection. C, detection of overexpressed Myc-DN-NRF-1 in nuclear extracts by Western blot analysis. 0.8 μg of pCDNA3.1 Myc-DN-NRF-1 or vector was transfected, and the nuclear extracts were obtained from the transfected cells. 25 μg of nuclear proteins were loaded into each well, and Myc-DN-NRF-1 was detected with anti-Myc antibody.