FIGURE 2.

IKKβ^ΔNBD associates with endogenous IKKα but not with endogenous NEMO. IKK complexes in whole cell lysates of WT, IKKβ^−/− (β^−/−), IKKβ^WT (β^WT), and IKKβ^ΔNBD (β^ΔNBD) MEFs were immunoprecipitated (IP) using anti-NEMO (A), anti-IKKα (B), or anti-IKKβ (C). Immunoprecipitated material was immunoblotted using anti-IKKα, anti-IKKβ, and anti-NEMO as indicated (right). Samples of lysates saved prior to immunoprecipitation (Pre-IP) were immunoblotted using anti-IKKα, anti-IKKβ, and anti-NEMO as shown.