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Effects of mutating Thr221 on the cADPR hydrolysis and NGD cyclization activities of CD38
| CD38 | cADPR→ADPR Va | NGD→cGDPR |
|
|---|---|---|---|
| Vmax | Km | ||
| nmol/mg/min | nmol/mg/min | μm | |
| WT | 6,000 ± 2,000 | 31,000 ± 400 | 5 ± 1 |
| T221F/E146A | 0 | 100,000 ± 10,000 | 60 ± 10 |
| T221F | 500 ± 20 | 5,000 ± 90 | 10 ± 1 |
aActivities were determined only at 1 mm cADPR, because the activities of the mutants were too low for Vmax/Km determination.
