FIGURE 1.

Identification and CCV enrichment of clavesin proteins. A, alignment of clavesin 1 and 2. Identical amino acids are shaded black. The dashed underline indicates the Sec14 domain, and the solid underline indicates the CRAL_TRIO_N portion of the Sec14 domain. The box denotes a clathrin box. B, the level of clavesin 1 and 2 in the indicated tissues was determined by Western blot. C, rat brain subcellular fractions from a preparation leading to highly enriched CCVs were blotted with clavesin 1/2 and CHC antibodies. H, homogenate; P, pellet; S, supernatant; SGp, sucrose gradient pellet; SGs, sucrose gradient supernatant.