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Clavesins co-localize with clathrin coat components. Hippocampal neurons at 21 DIV were processed by indirect immunofluorescence with polyclonal antibody against clavesin (red) and monoclonal antibodies against AP-1 (A) and CHC (B), each in green. In each case, the lower panels are from the area indicated by the white box in the merge images. The arrows point to co-localizing structures. Bars, 10 and 2 μm for the low and high power images, respectively.
