FIGURE 4.

Interaction of purified maspin and α5β1-Fc fusion proteins. a, recombinant maspin (10, 50, 100, and 250 pmol), in the presence or absence of reduction with DTT, was run on SDS-PAGE, and blots subsequently incubated with α5β1-Fc fusion protein (1 μg/ml) and probed with an anti-β1 antibody. b, α5β1-Fc (5, 10, and 30 pmol), in the presence or absence of reduction with DTT, was run on SDS-PAGE, and blots subsequently incubated with maspin (1 μg/ml) and probed with an anti-maspin antibody. Each panel also shows control blots, in which incubation with either α5β1-Fc or maspin, respectively, has been omitted.