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. Author manuscript; available in PMC: 2009 Nov 30.
Published in final edited form as: Mol Immunol. 2008 Feb 13;45(9):2651–2659. doi: 10.1016/j.molimm.2007.12.013

Figure 1.

Figure 1

Figure 1

Tetramer Guided Epitope Mapping of Infuenza B epitopes. (A) Pool Mapping: T cells from a DR1401 donor were stimulated with overlapping peptide mixtures spanning Flu B HA and stained using pooled peptide loaded tetramers after two weeks. Peptide pools 3, 7, and 8 gave positive staining. (B) Individual peptide mapping: T cells from the tetramer positive panels shown in A were stained again using tetramers loaded with individual peptide from the corresponding peptide pools. Peptides p14, p15, p33, p34, and p40 were identified as peptides containing DR1401 restricted epitopes.