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Mono and dinucleotide deletion mutations within polyA repeat region in the EGFR 3’UTR. A. Representative chromatograms from wild type and deletion mutant cell lines. B. Validation of direct sequencing of EGFR3’UTR by fragment analysis. The most dominant peak represents the true fragment length. The first peak to the right of the dominant peak represents an adenine addition. Other peaks likely represent polymerase slippage.
