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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cancer Res. 2009 Sep 29;69(19):7811–7818. doi: 10.1158/0008-5472.CAN-09-0986

Figure 5.

Figure 5

Single and dinucleotide deletion mutations within the polyA repeat of the EGFR 3’UTR increase mRNA stability. HCT116 cells were transiently transfected with pTRE-luciferase-EGFR 3’UTR A13 (WT), pTRE-luciferase-EGFR 3’UTR A12, or pTRE-luciferase-EGFR 3’UTR A11 in combination with the Tet-off vector, and the TK-Renilla transfection efficiency control. Twelve hours post transfection, doxycycline was added to a final concentration of 25 ng/ml. Cells were harvested at 24 hours and Luciferase activity determined. Values shown are the percentage of firefly luciferase in doxycycline treated cells relative to untreated cells. All values are corrected for TK-Renilla luciferase activity. Values shown are mean ± SEM from a representative experiment performed in triplicate (*P<0.05, **P<0.0005).