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. 2009 Nov 30;106(49):20770–20775. doi: 10.1073/pnas.0909812106

Fig. 3.

Fig. 3.

Apical constriction is not due to inhibiting endocytosis, and dynamin is enriched at the apical junctional region. (A) Maximum intensity projection images of cells expressing Dyn2wt-GFP (Left Upper, green) and Dyn2K44A-GFP (Left Lower, green) that were incubated with TxRed-transferrin (red) and stained for ZO-1 (blue). (B) Single plane images taken below AJC of Dyn2K44A-GFP-expressing cells subjected to TxRed-dextran applied either apically or basolaterally and imaged live. (C) Mean ratio (AP/L) for cells expressing Dyn2wt-GFP, Dyn2K44A-GFP, Eps15wt-GFP, Eps15Δ95/295-GFP, FLAG-Cav1wt, FLAG-Cav1Y14F, or Eps15Δ95/295-GFP and FLAG-Cav1Y14F in at least three separate experiments. **, P < 0.001. (D) Mean ratio (AP/L) for cells expressing Dyn2wt-GFP, Dyn2K44A-GFP, Dyn2K44A-CeFP, and Eps15Δ95/295-GFP, or Dyn2K44A-GFP and FLAG-Cav1Y14F from two independent experiments. (E) Single-plane images of a monolayer stained for dynamin (green) and ZO-1 (red) at the AJC (Upper) and 1 μm below the AJC (Lower). Note dynamin enrichment (arrows) at the AJC but not at the basolateral membranes. (F) XZ sections of cells expressing Dyn2wt-GFP (Left) and Dyn2K44A-GFP (Right) before and after treatment with 0.01% digitonin. Note the enrichment of Dyn2K44A-GFP relative to Dyn2wt-GFP (arrows). (G) FRAP recovery kinetics for Dyn2wt-GFP and Dyn2K44A-GFP in cells expressing an individual chimera.