Retention of Brd4 on mitotic chromosomes: reloading of Pol II and P-TEFb on chromosomes after telophase. Synchronized NIH3T3 cells were allowed to proceed through the indicated mitotic stages, fixed with paraformaldehyde, permeabilized, and immunostained for (A) Brd4 and Pol II, (B) Cdk9, and (C) Cyclin T1. Cells were coimmunostained for lamin B and counterstained for DNA with Hoechst 33342.