Figure 4.

Reduction of postmitotic de novo transcription in Brd4 knockdown cells. (A) Cells synchronized as in Figure 3A were allowed to reach telophase, pulsed with BrU for 15 min and immunostained for Brd4 (green) and BrU (red), counterstained for DNA. (B) Control and Brd4 knockdown cells synchronized as above were pulsed with [³H]uridine for 15 min and amounts of incorporated ³H were quantified. The values represent the average of three assays ± SD. (C) Control and Brd4 knockdown cells synchronized as above were tested for de novo transcription of indicated genes by qRT-PCR. Transcript levels were normalized by 18S RNA. Values represent the average of three determinations ± SD. Results of additional genes are shown in Supplementary Figure S2. The primer information is in Supplementary Table S1.