Skip to main content
. 2009 Dec 1;20(23):4941–4950. doi: 10.1091/mbc.E09-03-0200

Figure 4.

Figure 4.

The percentage of Ki67+ NPCs is increased by overexpression of RhoG-V12 and decreased by knockdown of RhoG in vivo. (A) Brains were fixed at E16 after electroporation with control or RhoG-V12 vector at E14, and cortical sections were stained with anti-Ki67 (red), anti-GFP (green), and Hoechst 33258 (blue). Scale bar, 50 μm. (B) Quantification of the percentages of Ki67+ cells. Control, n = 3; RhoG-V12, n = 3. Values indicate means ± SEM; ***p < 0.001 versus control (Student's t test). (C) Brains were fixed at E16 after electroporation with shLuc, shRG-A, -B, or shRG-A together with human wild-type RhoG (hRhoG-WT) at E14 and stained with anti-Ki67 (red), anti-GFP (green), and Hoechst 33258 (blue). Scale bar, 50 μm. (D) Quantification of the percentages of Ki67+ cells. shLuc, n = 3; shRG-A, n = 3; shRG-B, n = 3; shRG-A + hRhoG-WT, n = 3. Values indicate means ± SEM; *p < 0.05 versus shLuc (Student's t test).