Figure 5.

Knockdown of RhoG does not induce apoptosis. (A) Brains were fixed at E16 after electroporation with shLuc, shRG-A, or s-B vector at E14, and TUNEL assays were performed (red, middle). Cortical sections were stained with anti-GFP antibody (green, left). Scale bar, 100 μm. (B) Quantification of the numbers of TUNEL⁺ cells per the area of 10,000 μm² of equally transfected cortical sections. shLuc, n = 3; shRG-A, n = 3; shRG-B, n = 3. Values indicate means ± SEM. (C) For data validation, shLuc-electroporated sections were pretreated with DNase I as a positive control. Scale bar, 20 μm.