Skip to main content
. 2009 Dec 1;20(23):4962–4975. doi: 10.1091/mbc.E09-08-0712

Figure 1.

Figure 1.

Down-regulation of both Munc18-1 and Munc18-2 results in dramatic reductions in syntaxin-1 expression and NE secretion. (A) Expression of Munc18-2 in rat adrenal glands and wild-type PC12 cells. Twenty micrograms of total homogenates from the rat adrenal glands and wild-type PC12 cells was analyzed by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblotting using anti-Munc18-2 antibodies. The signal was detected with enhanced chemiluminescence detection system. A number on the left indicates the position of a molecular weight marker. (B) Control (C1) and Munc18-1 knockdown (KD43) clones [isolated in Arunachalam et al. (2008)] were infected with lentiviruses generated from pLKO-neo (control) or pLKO-Munc18-2 knockdown plasmid. The infected cells were selected with neomycin (0.8 mg/ml). Thirty micrograms of total homogenates from heterologous pools of the surviving cells was analyzed by SDS-PAGE and immunoblotting using anti-Munc18-1 and -2 and syntaxin-1, -2, and -3 antibodies. (C) NE release was stimulated by 70 mM KCl for 15 min from heterologous pools of control, Munc18-1 single knockdown, Munc18-2 single knockdown, and Munc18-1/-2 double knockdown cells. Error bars indicate SEM (n = 15).